Protein analytics
Prof. Dr. Klaus Lingelbach
Fachbereich Biologie
Karl-von-Frisch-Straße
35032 Marburg
Phone: +49-6421-2823404
(Lingelbach)
Fax: +49-6421-2821531
(Lingelbach)
e-mail: Lingelba@staff.uni-marburg.de
Dr. Stefan Baumeister
FB Biologie, Protein Analytik
Karl von Frisch Strasse 8
35032 Marburg
Fon : +49-6421-2823421 or 2823364
Fax : +49-6421-2821531
Email: baumeist@staff.uni-marburg.de
Kern B Third floor room 3341 or 3337
Service:
Protein identification: proteolytic in-gel digest (or in-solution
digest), followed by MALDI-TOF-MS peptide fingerprints and MS/MS
analyses combined with database searches.
Equipment:
#Isoelectric-focusing systems (1st dimension):
(i) IPGphore (GE Healthcare); (ii) PROTEAN IEF Cell (Biorad); (iii)
Micro-Rotofor Cell for in solution IEF (Biorad)
(i) (II)
(iii)
#Electrophoresis systems (2nd dimension):
(i) XCell Mini-Gel (Invitrogen); (ii) Prot-Resolv Mini-LC and
Maxi-LC (Phase); (iii) Ettan Dalt (GE Healthcare)
(i) (ii)
(iii)
#Protein extraction: (i) Whole Gel Eluter
(Biorad)
(i)
#Preparation and MALDI analyses:
(i) PROTEINEER spII Spot-picker (Bruker Daltonics), (ii) a
PROTEINEER dp liquid handling system for automatic proteolytic in-gel
digest (Bruker Daltonics) and (iii) a Ultraflex TOF/TOF MALDI
instrument (Bruker Daltonics).
(i) (ii)
(iii)
#LC-MALDI analyses:
(i) nano-HPLC chromatography system Ultimate 3000 (Dionex) coupled
to a fraction collector PROTEINEER fc (Bruker Daltonics) for direct
spotting of complete LC-runs on a MALDI-TOF target.
(i)
#2D analysing Software:
(i) Delta2D (Decodon) for aligning 2D-gel images by (automatic)
warping, thus eliminating differences in spot position. Warped images
are then overlaid to produce dual channel images with clearly visible
qualitative and quantitative differences in expression levels.
#Fluorescence-Laser-Scanner:
(i) Typhoon Trio (GE Healthcare) for 2-D
Fluorescence Difference Gel Electrophoresis (DIGE) + ImageQuant TL
software for analysis of 1D / 2D gel images and Decyder software for
the analysis of 2D DIGE images
(i)
Staff:
Braun, Nadja – Chemielaborantin; -2823364
