BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Plone.org//NONSGML plone.app.event//EN
X-WR-TIMEZONE:Europe/Berlin
BEGIN:VEVENT
SUMMARY:Prof. Tung Le\, Centre for Microbial Interactions\, Norwich: The s
 ilence of the genes: How a molecular clamp shuts down long-range gene expr
 ession in multi-drug resistance plasmids?
DTSTART;TZID=Europe/Berlin:20250415T090000
DTEND;TZID=Europe/Berlin:20250415T100000
DTSTAMP:20260409T110714Z
UID:73359b7be02c44abbad8c2b6a000bb9f@www.uni-marburg.de
CREATED:20241205T125047Z
DESCRIPTION:https://www.uni-marburg.de/en/synmikro/grk2937/whats-on/dates-
 and-events/grk2937_speaker-series_tung-le\nSpeaker Series. Long-range gene
  regulation in bacteria is rare and typically attributed to DNA looping an
 d supercoiling. In this study\, we use a combination of biophysical approa
 ches—including X-ray crystallography and single-molecule analysis—to i
 nvestigate the KorB–KorA system in Escherichia coli. Our findings reveal
  that long-range gene silencing on the plasmid RK2\, a key driver of multi
 -drug resistance in diverse Gram-negative bacteria\, is facilitated cooper
 atively by the DNA-sliding clamp KorB and the clamp-locking protein KorA. 
 We demonstrate that KorB functions as a CTPase clamp capable of entrapping
  and sliding along DNA to reach target promoters up to 1.5 kb away. By res
 olving the tripartite crystal structure of the KorB–KorA–DNA complex\,
  we show that KorA locks KorB into a closed clamp state. When bound to DNA
 \, KorA enhances repression by halting KorB’s sliding at target promoter
 s\, thereby blocking RNA polymerase holoenzyme access. Overall\, our study
  elucidates the molecular mechanism underlying KorB’s dual role as a DNA
 -sliding clamp and a co-repressor\, offering an alternative model for long
 -range gene regulation in bacteria. In the final part of my talk\, I will 
 discuss the evolutionary adaptation of CTPase enzymes to perform a wide ra
 nge of biological functions.
LAST-MODIFIED:20250317T104949Z
LOCATION:SYNMIKRO Lecture Hall
URL:https://www.uni-marburg.de/en/synmikro/grk2937/whats-on/dates-and-even
 ts/grk2937_speaker-series_tung-le
END:VEVENT
BEGIN:VTIMEZONE
TZID:Europe/Berlin
X-LIC-LOCATION:Europe/Berlin
BEGIN:DAYLIGHT
DTSTART:20250330T030000
TZNAME:CEST
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
END:DAYLIGHT
END:VTIMEZONE
END:VCALENDAR