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Automation platform
Daniel Schindler, Hendrik Runkel
Complementing molecular biology and synthetic biology technologies, the aim is to establish a platform that allows for the standardized management of DNA elements and the automation of their assembly. This includes the development of DNA assembly routines and the establishment of high-throughput methods for the cultivation and characterization of C. reinhardtii.
The MaxGENESYS DNA Foundry provides the infrastructure to develop this automation and make it available to all RobuCop members, external collaboration partners, and industry. The primary goal is the assembly, validation, characterization, and cataloging of synthetic DNA constructs.
An archive for the physical samples (DNA constructs and cryocultures) is being established using 2D barcode-encoded archiving systems, and a system for automated management is being developed. For high-throughput cultivation methods with C. reinhardtii, an incubator with an adjustable light and CO2 module is available, enabling the parallel incubation of up to 24 deep-well plates at constant light intensity.Automated, image-based analysis routines for high-throughput are being established for this purpose.
