Prof. Dr. Johann Heider
Original Publications
Schühle, K. & Heider, J. (2012) Acetone and butanone metabolism of the denitrifying bacterium Aromatoleum aromaticum demonstrates novel biochemical properties of an ATP-dependent aliphatic ketone carboxylase. J. Bacteriol. 194, 131-141.
H ilberg, M., Pierik, A. J., Bill, E., Friedrich, T., Lippert, M.-L., & Heider, J. (2012) Identification of FeS clusters in the glycyl-radical enzyme benzylsuccinate synthase via EPR and Mössbaur spectroscopy. J. Biol. Inorg. Chem. 17, 49-56.
Szaleniec M., Salwinski A., Borowski T., Heider J. & Witko M. (2011) Quantum chemical modeling studies of ethylbenzene dehydrogenase activity. Intl. J. Quant. Chem. DOI 10.1002/qua23143.
Stöveken, N., Pittelkow. M., Sinner, T., Jensen, R.A., Heider, J. & Bremer, E. (2011) A specialized aspartokinase enhances the biosynthesis of the osmoprotectans ectoine and hydroxyectoine in Pseudomonas stutzeri A1501. J. Bacteriol. 17, 4456-4468.
Rabus, R., Jarling, R., Lahme, S., Kühner, S., Heider, J., Widdel, F. & Wilkes, H. (2011) Co-metabolic conversion of toluene in anaerobic n-alkane-degrading bacteria. Environ. Microbiol., 9, 2576-2586.
Jobst B., Schühle K., Linne U. & Heider J. (2010) ATP-Dependent Carboxylation of Acetophenone by a Novel Type of Carboxylase. J. Bacteriol. 192, 1387-1394Szaleniec M., Borowski T., Schühle K., Witko M. and Heider J. (2010) Ab initio modelling of ethylbenzene dehydrogenase reaction mechamism. J. Amer. Chem. 132, 6014-6024.
Müller F. D., Treuner-Lange A., Heider J., Huntley S. M., Higgs P. I. (2010) Global transcriptome analysis of spore formation in Myxococcus xanthus reveals a locus necessary for cell differentiation. BMC Genomics11, 264
Szaleniec M., Witko M & Heider J. (2008) Quantum chemical modelling of C-H cleavage mechanism in oxidation of ethylbenzene and its derivates by ethylbenzene dehydrogenase. J. Molec. Catal. A, 286, 128-136
Grundmann O., Behrends A., Rabus R., Amann J., Halder T., Heider J. & Widdel F. (2008) Genes encoding a tentative enzyme for anaerobic activation of n-alkanes in the denitrifying bacterium, strain HxN1. Environ. Microbiol., 10, 376-385
Breuer M., Rabus R. and Heider J. (2008) Method for production of optically active alcohols using Azoarcus sp. EbN1 dehydrogenase. Pub. No. WO/2008/1545302; Intl. Appl. No. PCT/EP2008/057522
Szaleniec M., Hagel C., Menke M., Nowak P., Witko M. & Heider J. (2007) Kinetics and mechanism of oxygen-independent hydrocarbon-hydroxylation by ethylbenzene dehydrogenase. Biochemistry 46, 7637-7646
Kloer D. P., Hagel C., Heider J. & Schulz, G. E. (2006) Crystal Structure of Ethylbenzene dehydrogenase from Aromatoleum aromaticum. Structure 14, 1377-1388
Höffken H. W., Duong M., Friedrich T., Breuer M., Hauer B., Reinhardt R., Rabus R., Heider J. (2006) Crystal structure and enzyme kinetics of the (S)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain EbN1. Biochemistry, 45, 82-93
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F. & Reinhard R. (2005) The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1. Arch. Microbiol. 183, 27-36
Verfürth K., Pierik A. J., Leutwein C., Zorn S. & Heider J. (2004) Substrate specificities and electron paramagnetic resonance properties of benzylsuccinate synthases in anaerobic toluene- and m-xylene-metabolism. Arch. Microbiol. 181, 155-162
Kube M., Heider J., Amann J., Hufnagel P., Kühner S., Beck A., Reinhardt R. & Rabus R. (2004) Genes involved in the anaerobic degradation of toluene in a denitrifying bacterium, strain EbN1. Arch. Microbiol. 181, 182-194
Szaleniec M., Jobst B. & Heider J. (2003) Ethylbenzene dehydrogenase: oxidation of hydrocarbons without oxygen. Ann. Pol. Chem.Soc. 2003, 240-245
Duboc-Toia C., Hassan A. K., Mulliez E., Ollagnier-de Choudens S., Fontecave M., Leutwein C. & Heider J. (2003) Very high field EPR studies of glycyl radical enzymes. J. Amer. Chem. Soc. 125, 38-39
Hermuth K., Leuthner B. & Heider J. (2002) Operon structure and expression of the genes for benzylsuccinate synthase in Thauera aromatica strain K172. Arch. Microbiol. 177, 132-138
Mohamed M. E., Ismail W., Heider J. & Fuchs G (2002) Aerobic metabolism of phenylacetic acids in Azoarcus evansii. Arch. Microbiol. 178, 180-192
Leutwein C. & Heider J. (2002) (R)-Benzylsuccinyl-CoA dehydrogenase from Thauera aromatica: a new acyl-CoA dehydrogenase involved in anaerobic toluene catabolism. Arch. Microbiol. 178, 517-524
Leutwein C. & Heider J. (2001) Succinyl-CoA: (R)-benzylsuccinate CoA-transferase: an enzyme of the anaerobic toluene catabolic pathway in denitrifying bacteria. J. Bacteriol. 183, 4288-4295
Kniemeyer O. & Heider J. (2001) Ethylbenzene dehydrogenase, a novel hydrocarbon-oxidising molybdenum/iron-sulfur/heme enzyme. J. Biol. Chem. 276, 21381-21386
Kniemeyer O. & Heider J. (2001) (S)-1-Phenylethanol dehydrogenase of Azoarcus sp. strain EbN1, an enzyme of anaerobic ethylbenzene catabolism. Arch. Microbiol. 176, 129-135
Leuthner B. & Heider J. (2000) Anaerobic toluene catabolism of Thauera aromatica: a toluene-induced operon codes for the enzymes of ß-oxidation of the intermediate benzylsuccinate. J. Bacteriol. 182, 272-277
Zengler K., Heider J., Rosselló-Mora R. & Widdel F. (1999) Phototrophic utilisation of toluene under anoxic conditions by a new strain of Blastochloris sulfoviridis. Arch. Microbiol. 172, 204-212
Leutwein C. & Heider J. (1999) Anaerobic toluene catabolic pathway in denitrifying Thauera aromatica: activation and ß-oxidation of the first intermediate, (R)-(+)-benzylsuccinate. Microbiol. 145, 3265- 3271
Leuthner B., Leutwein C., Schulz H., Hörth P., Haehnel H., Schiltz E., Schägger H. & Heider J. (1998) Biochemical and genetic characterisation of benzylsuccinate synthase from Thauera aromatica: a new glycyl-radical enzyme catalysing the first step in anaerobic toluene metabolism. Molec. Microbiol. 28, 615-628
Heider J., Boll M., Breese K., Breinig S., Ebenau-Jehle C., Feil U., Gad’on N., Laempe D., Leuthner B., Mohamed M., Schneider S., Burchhardt G. & Fuchs G. (1998) Differential induction of enzymes involved in anaerobic metabolism of aromatic compounds in the denitrifying bacterium Thauera aromatica. Arch. Microbiol. 170, 120-131
Leuthner B. & Heider J. (1998) A two-component system involved in regulation of anaerobic toluene metabolism in Thauera aromatica. FEMS Microbiol. Lett. 166, 35-41
Rabus R. & Heider J. (1998) Initial reactions of anaerobic metabolism of alkylbenzenes in denitrifying and sulfate-reducing bacteria. Arch. Microbiol. 170, 377-384
Müller S., Heider J. & Böck A. (1997) The path of unspecific incorporation of selenium in Escherichia coli. Arch. Microbiol. 168, 421-427
Biegert T., Fuchs G. & Heider J. (1996) Evidence that anaerobic oxidation of toluene in the denitrifying bacterium Thauera aromatica is initiated by formation of benzylsuccinate from toluene and fumarate, Eur. J. Biochem. 238, 661-668
Heider J., Mai X. & Adams M. W. W. (1996) Characterisation of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A dependent enzyme involved in peptide fermentation by hyperthermophilic archaea. J. Bacteriol. 178, 780-787
Hüttenhofer A., Heider J. & Böck A. (1996) Interaction of the Escherichia coli fdhF hairpin promoting selenocysteine incorporation with the ribosome. Nucl. Acids Res. 24, 3903-3910
Ma K., Loessner H., Heider J., Johnson M. K. & Adams M. W. W. (1995) Effects of elemental sulfur on the metabolism of the deep-sea hyperthermophilic archaeon Thermococcus strain ES-1: characterisation of a sulfur-regulated, non-heme iron alcohol dehydrogenase. J. Bacteriol. 177, 4748-4756
Heider J., Ma K. & Adams M. W. W. (1995) Purification, characterisation and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Thermococcus strain ES-1. J. Bacteriol. 177, 4757-4764
Tormay P., Wilting R., Heider J. & Böck A. (1994) Genes coding for the selenocysteine-inserting tRNA species from Desulfomicrobium baculatum and Clostridium thermoaceticum: structural and evolutionary implications. J. Bacteriol. 176, 1268-1274
Baron C., Heider J. & Böck A. (1993) Interaction of translation factor SELB with the formate dehydrogenase H selenopolypeptide mRNA. Proc. Natl. Acad. Sci. USA 90, 4181-4185
Heider J. & Böck A. (1992) Targeted insertion of selenocysteine into the alpha-subunit of formate dehydrogenase from Methanobacterium formicicum. J. Bacteriol. 174, 659-663
Heider J., Baron C. & Böck A. (1992) Coding from a distance: dissection of the mRNA determinants required for the incorporation of selenocysteine into protein. EMBO J. 11, 3759-3766
Heider J., Forchhammer K., Sawers G. & Böck A. (1991) Interspecies compatibility of selenoprotein biosynthesis in Enterobacteriaceae. Arch. Microbiol. 155, 221-228
Sawers G., Heider J., Zehelein E. & Böck A. (1991) Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme. J. Bacteriol. 173, 4983-4993
Berg B. L., Li J., Heider J. & Stewart V. (1991) Nitrate-inducible formate dehydrogenase in Escherichia coli K-12: I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine. J. Biol. Chem. 266, 22380-22385
Zinoni F., Heider J. & Böck A. (1990) Features of the formate dehydrogenase mRNA necessary for decoding of the UGA codon as selenocysteine. Proc. Natl. Acad. Sci. USA 87, 4660-4664
Baron C., Heider J. & Böck A. (1990) Mutagenesis of selC, the gene for the selenocysteine-inserting tRNA-species in E. coli: effects on in vivo function. Nucl. Acids Res. 18, 6761-6766
Heider J., Leinfelder W. & Böck A. (1989) Occurrence and functional compatibility of a tRNA species which inserts selenocysteine into protein. Nucl. Acids Res. 17, 2529-2540
Reviews
Schühle K. & Heider J. (2012) Anaerobic biodegradation of hydrocarbons including methane. In:The prokaryotes-a handbook on the biology of bacteria, 4th ed. (Rosenberg E., DeLong E.F., Thompson F., Lory S., Stackebrandt E. eds), accepted, Springer-Verlag, Heidelberg
Fuchs, G., Boll, M. and Heider, J. (2011) Microbial degradation of aromatic compounds – from one strategy to four. Nat Rev Microbiol,9, 803-816.
Boll M. & Heider J. (2009) Anaerobic Degradation of Hydrocarbons: Mechanisms of C-H-bond activation in the absence of oxygen. In. Handbook of Hydrocarbon and Lipid Microbiology (ed. K. N. Timmis), Springer-Verlag, Heidelberg
Heider J. (2008) Bakterieller Kohlenwasserstoff-Abbau—es geht auch ohne Sauerstoff. Biospektrum 14, 28-31
Heider J. & Rabus R. (2007) Genomic insights in the anaerobic biodegradation of organic pollutants. In: Microbial biodegradation: Genomics and molecular biology (E. Diaz ed.), pp. 25-54, Caister academic press, Norfolk, UK
Heider J. (2007) Adding handles to unhandy substrates: anaerobic hydrocarbon activation mechanisms. Curr. Opin. Chem. Biol. 11, 188-194
Selmer T., Pierik A. J., Heider J. (2005) New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria. Biol. Chem. 386, 981-988
Heider J. & Fuchs G. (2005) Genus Thauera. In: Bergey’s Manual of Systematic Bacteriology (2nd ed); Vol. 2, part C (G. Garitty, ed.), pp. 907-913, Springer Verlag, Berlin
Boll M., Fuchs G. & Heider J. (2002) Anaerobic oxidation of aromatic compounds and hydrocarbons. Curr. Opin. Chem. Biol. 6, 604-611
Heider J. (2001) A new family of CoA-transferases. FEBS Lett. 509, 345-349
Heider J. (2000) Toluol-Stoffwechsel ohne Sauerstoff: eine (Glycyl-)radikale Lösung. Biospektrum 6, 280-281
Heider J., Spormann, A. M., Beller H. R. & Widdel, F. (1999) Anaerobic bacterial metabolism of hydrocarbons. FEMS Microbiol. Rev. 22, 459-473
Heider J. & Fuchs G. (1997) Anaerobic metabolism of aromatic compounds, Eur. J. Biochem. 243, 577-596
Heider J. & Fuchs G. (1997) Microbial anaerobic aromatic metabolism, Anaerobe 3, 1-22
Heider J. & Böck A. (1993) Selenium metabolism in micro-organisms. Adv. Microb. Physiol. 35, 71-109
Böck A., Forchhammer K., Heider J., Leinfelder W., Sawers G., Veprek B. & Zinoni F (1991) Selenocysteine: the 21st amino acid. Molec. Microbiol. 5, 515-520
Böck A., Forchhammer K., Heider J. & Baron C. (1991) Selenoprotein synthesis: an expansion of the genetic code. Trends Biochem. Sci. 16, 463-467
Böck A., Baron C., Forchhammer K., Heider J., Leinfelder W., Sawers G., Veprek B., Zehelein E. & Zinoni F. (1990) From nonsense to sense: UGA encodes selenocysteine in formate dehydrogenase and other selenoproteins. In: 41. Colloquium Mosbach: The molecular basis of bacterial metabolism (G. Hauska & R. Thauer eds.), pp. 61-68, Springer Verlag, Berlin
Textbook chapters
Heider J. (2006) Oxidation anorganischer Verbindungen: Chemolithotrophe Lebensweise. In: Allgemeine Mikrobiologie, 8. Aufl. (G. Fuchs ed.), pp. 321-346, Thieme Verlag, Stuttgart
Heider J. (2006) Mikrobielle Gärungen. In: Allgemeine Mikrobiologie, 8. Aufl. (G. Fuchs ed.), pp. 347-378, Thieme Verlag, Stuttgart
Heider J. (2006) Anaerobe Atmung. In: Allgemeine Mikrobiologie, 8. Aufl. (G. Fuchs ed.), pp. 379-404, Thieme Verlag, Stuttgart
