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Dr. Mariana Sanchez Londoño

Postdoctoral Researcher, WG Randau
Biology, Philipps-Universität Marburg
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Project: Live-cell single-molecule visualization of the Type IV-A CRISPR-Cas interference mechanism

Project summary:
Building on my expertise in Type IV CRISPR-Cas systems, my proposed postdoctoral research aims to develop CRISPR-based gene regulation tools for studying gut microbiome dynamics. In collaboration with Victor Sourjik’s group, this project aims to optimize Type IV CRISPRi to downregulate chromosomal genes in long operons within Bacteroides spp., key players in gut health. Additionally, I will explore engineered nuclease-helicase fusion proteins for controlled genomic modifications. Expanding on my SMM analyses, I will integrate advanced imaging techniques with synthetic CRISPR RNAs to achieve high spatial and temporal resolution in CRISPR-mediated regulation of specific microbiome members. My ultimate goal is to establish a foundation for an independent research line, starting with CRISPRi optimization for microbial community regulation and its implications for human health.

Cooperation with: Victor Sourjik (MPI-TM)

Cooperation Partners