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Ismath Sadhir 

Doctoral Researcher, RG Murray
MPI for Terrestrial Microbiology
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Project: Establishment of a high-throughput single-cell approach combined with single molecule tracking to study bacterial chromosome organization

Project summary:
In this project, I will study the spatiotemporal organization of the Bacillus subtilis chromosome during its cell cycle. Through a high-throughput single-cell approach based on microfluidic devices called mothermachines, I will determine the dynamics of several fluorescently labelled chromosomal regions across thousands of cell cycles. This will provide a definitive understanding of the organization and choreography of the replicating chromosome. I will also couple the microfluidic approach with super resolution microscopy to study the spatiotemporal dynamics of the condensin complex and thereby dissect its role in chromosome organization. The combination of these two powerful techniques has enormous potential and will open the way for many future studies.

Cooperation with: AG Graumann (SYNMIKRO, UMR)

Cooperation Partners