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Research area and techniques

G protein mediated signal transduction

Spatio-temporal organisation of G protein mediated signaling pathways

• Kinetics of receptor activation, receptor/G protein interaction, G protein activation , G protein/effector interaction
• Distribution of receptors G proteins and effectors into membrane microdomains and their impact on dynamics of GPCR signaling (SFB593 TP A13)
• Voltage dependence of receptor activation
• Regulation of receptor function by arrestin

Imaging of G protein signalling in intact tissues

• Real time cAMP imaging by means of FRET in tissues from transgenic mice
• Generation of mice with tissue specific expression of FRET-based biosensors

Experimental techniques

• Fluorescence microscopy
• Förster (fluorescence) resonance energy transfer (FRET)
• Total internal reflection microscopy (TIRF)
• Patch clamp recording
• Cell culture and transfection
• Molecular biology, cloning
• Immunhistochemistry, immunoblots, immunprecipitation, confocal laser scanning microscopy
• RNAi-technology