Research area and techniques

G protein mediated signal transduction

Spatio-temporal organisation of G protein mediated signaling pathways

  • Kinetics of receptor activation, receptor/G protein interaction, G protein activation , G protein/effector interaction
  • Distribution of receptors G proteins and effectors into membrane microdomains and their impact on dynamics of GPCR signaling (SFB593 TP A13)
  • Voltage dependence of receptor activation
  • Regulation of receptor function by arrestin

Imaging of G protein signalling in intact tissues

  • Real time cAMP imaging by means of FRET in tissues from transgenic mice
  • Generation of mice with tissue specific expression of FRET-based biosensors

Experimental techniques

  • Fluorescence microscopy
  • Förster (fluorescence) resonance energy transfer (FRET)
  • Total internal reflection microscopy (TIRF)
  • Patch clamp recording
  • Cell culture and transfection
  • Molecular biology, cloning
  • Immunhistochemistry, immunoblots, immunprecipitation, confocal laser scanning microscopy
  • RNAi-technology