PD Dr. Jude Przyborski

Contact Jude Przyborski FB Biologie Parasitologie Karl von Frisch Strasse 35043 Marburg Germany E-mail: przybors(at)staff.uni-marburg(dot)de Tel: +49-6421-2826596

Group Members

Dr. Simone Külzer (exported Hsp40s),

Pradipta Mandal (PhD Student, P. falciparum chaperone networks),

Irine Bietz (PhD Student, Apicoplast Transport),

Luis Barniol (PhD Student, P. falciparum signal sequence)

Sabrina Heiny (PhD Student, Apicoplast Transport)

Project description

My work concentrates on various topics related to protein trafficking mechanisms in the human malarial parasite P. falciparum. Techniques used include cell fractionation, proteomics, bioinformatics as well as recombinant DNA technology and transfection of blood stage P. falciparum. Our eventual aim is to characterise the trafficking pathways, and machinery of this intracellular parasite, with a view to identifying targets for intervention strategies.

A GFP fusion protein targeted to the Maurer's clefts of P. falciparum infected human erythrocytes (from Przyborski et al, EMBO journal 2005)

                                                       

Trafficking of a GFP-fusion protein through the ER of P. falciparum can be stopped by either a) Addition of a -KDEL ER retention signal, or b) Treatment of the cells with brefeldin A (BFA) (From Przyborski et al EMBO journal 2005)

Opportunities

I also occasionally have opportunities for highly motivated BSc and MSc students. Just get in touch well ahead of time (we're quite popular, and get many requests) to request more information. PhD or Post-doctoral candidates with scholarships/ fellowships are welcome to contact me.

You can also apply for the Short Course for Young Parasitologists that I run for the German Society of Parasitology (Deutsche Gesellschaft für Parasitologie). Details can be found here: www.pfalciparum.de

Short CV

Habilitation in Cell Biology and Parasitology, 2010

Since April 2005 in Marburg

PhD-Trafficking of STEVOR to the Maurer's clefts of P. falciparum infected erythrocytes.
Liverpool School of Tropical Medicine, UK/ University of Heidelberg, Germany.

BSc (Hons)-Human Science.
University College London, UK

Collaborations

Marburg:       Uwe Maier (Protein transport)

Aachen:        Gabi Pradel (Regulation of gametogenesis)

Giessen:       Katja Becker (Protein trafficking)

Berlin:          Alyssa Ingmundson (Exported Hsp40s)

Montpellier:   Catherine Braun-Breton (Protein analysis)

Melbourne:    Geoff McFadden (Apicoplast transport)

                    Greg Blatch (Plasmodium chaperones)

                    Alex Maier (Exported Hsp40s)

                    Brendan Crabb / Paul Gilson (Protein translocation to the erythrocyte)

South Africa: Addmore Shonhai (Plasmodium chaperones)

Funding

DFG PR1099/1-1 (Protein transport to the infected erythrocyte), PR1099/1-2/SFBTR1 (Apicoplast transport), LI-12/1 (Chaperone networks in the malaria parasite), SPP 1580 (exported Hsp40 proteins)

Research publications and reviews

2011

Cockburn IL, Pesce ER, Pryzborski JM, Davies-Coleman MT, Clark PG, Keyzers RA, Stephens LL, Blatch GL. Screening for small molecule modulators of Hsp70 chaperone activity using protein aggregation suppression assays: inhibition of the plasmodial chaperone PfHsp70-1. Biol Chem. 2011 May;392(5):431-8. doi: 10.1515/BC.2011.040. Epub 2011 Mar 23.

Chu T, Lingelbach K, Przyborski JM. Genetic evidence strongly support an essential role for PfPV1 in intra-erythrocytic growth of P. falciparum. PLoS One. 2011 Mar 31;6(3):e18396.

Blatch GL, Przyborski JM. Protein biochemistry: don't forget the cell biology. Biochim Biophys Acta. 2011 Mar;1814(3):456.

Agarwal S, Kern S, Halbert J, Przyborski JM, Baumeister S, Dandekar T, Doerig C, Pradel G. Two nucleus-localized CDK-like kinases with crucial roles for malaria parasite erythrocytic replication are involved in phosphorylation of splicing factor. J Cell Biochem. 2011 May;112(5):1295-310. doi: 10.1002/jcb.23034.

Botha M, Chiang AN, Needham PG, Stephens LL, Hoppe HC, Külzer S, Przyborski JM, Lingelbach K, Wipf P, Brodsky JL, Shonhai A, Blatch GL. Plasmodium falciparum encodes a single cytosolic type I Hsp40 that functionally interacts with Hsp70 and is upregulated by heat shock. Cell Stress Chaperones. 2011 Jul;16(4):389-401. Epub 2010 Dec 30.

Shonhai A, Maier AG, Przyborski JM, Blatch GL. Intracellular protozoan parasites of humans: the role of molecular chaperones in development and pathogenesis. Protein Pept Lett. 2011 Feb;18(2):143-57. Review.

2010

Kehr S, Sturm N, Rahlfs S, Przyborski JM, Becker K. Compartmentation of redox metabolism in malaria parasites. PLoS Pathog. 2010 Dec 23;6(12):e1001242.

Külzer S, Rug M, Brinkmann K, Cannon P, Cowman A, Lingelbach K, Blatch GL, Maier AG, Przyborski JM. Parasite-encoded Hsp40 proteins define novel mobile structures in the cytosol of the P. falciparum-infected erythrocyte. Cell Microbiol. 2010 Oct;12(10):1398-420. doi: 10.1111/j.1462-5822.2010.01477.x.

Urscher M, Przyborski JM, Imoto M, Deponte M. Distinct subcellular localization in the cytosol and apicoplast, unexpected dimerization and inhibition of Plasmodium falciparum glyoxalases. Mol Microbiol. 2010 Apr;76(1):92-103. Epub 2010 Feb 10.

Baumeister S, Winterberg M, Przyborski JM, Lingelbach K. The malaria parasite Plasmodium falciparum: cell biological peculiarities and nutritional consequences. Protoplasma. 2010 Apr;240(1-4):3-12. Epub 2009 Nov 25. Review.

 

2009

Spork S, Hiss JA, Mandel K, Sommer M, Kooij TW, Chu T, Schneider G, Maier UG, Przyborski JM. An unusual ERAD-like complex is targeted to the apicoplast of Plasmodium falciparum. Eukaryot Cell. 2009 Aug;8(8):1134-45. Epub 2009 Jun 5.

Bietz S, Montilla I, Külzer S,Przyborski JM and Lingelbach K. Recruitment of human aquaporin 3 to internal membranes in the Plasmodium falciparum infected erythrocyte. Mol. Biochem. Para. 2009 Sep;167(1):48-53. Epub 2009 Apr 23.

Külzer S, Gehde N, andPrzyborski JM. Return to sender: use of Plasmodium ER retrieval sequences to study protein transport in the infected erythrocyte and predict putative ER protein families. Parasitology Research 2009 Jun; 104(6):1535-41. Epub 2009 Mar 18.

Gehde N, Hinrichs C, Montilla I, Charpian S, Lingelbach K and Przyborski JM. Protein unfolding is an essential requirement for transport across the parasitophorous vacuolar membrane of Plasmodium falciparum. Mol Micro. 2009 Feb;71(3):613-28. Epub 2008 Nov 24.

 

 

Hiss JA, Przyborski JM, Schwarte F, Lingelbach K, Schneider G. The Plasmodium export element revisited. PLoS ONE. 2008 Feb 6;3(2):e1560.

Charpian S, Przyborski JM. Protein transport across the parasitophorous vacuole of Plasmodium falciparum: into the great wide open. Traffic. 2008 Feb;9(2):157-65.

Hempel F, Bozarth A, Sommer MS, Zauner S, Przyborski JM, Maier UG. Transport of nuclear-encoded proteins into secondarily evolved plastids. Biol Chem. 2007 Sep;388(9):899-906.

Sommer MS, Gould SB, Lehmann P, Gruber A, Przyborski JM, Maier UG. Der1-mediated preprotein import into the periplastid compartment of chromalveolates? Mol Biol Evol. 2007 Apr;24(4):918-28.

Lingelbach K, Przyborski JM. The long and winding road: protein trafficking mechanisms in the Plasmodium falciparum infected erythrocyte. Mol Biochem Parasitol. 2006 May;147(1):1-8.

Viebig NK, Gamain B, Scheidig C, Lepolard C, Przyborski J, Lanzer M, Gysin J, Scherf A. (2005) A single member of the Plasmodium falciparum var multigene family determines cytoadhesion to the placental receptor chondroitin sulphate A. EMBO Rep. 2005 Jul 15

Przyborski JM, Miller SK, Pfahler JM, Henrich PP, Rohrbach P, Crabb BS,Lanzer M. (2005) Trafficking of STEVOR to the Maurer's clefts in Plasmodium falciparum-infected erythrocytes. EMBO journal Jul 6;24(13):2306-17.

Przyborski JM, Lanzer M. (2005) Protein transport and trafficking in Plasmodium falciparum-infected erythrocytes. Parasitology. Apr;130(Pt 4):373-88.

Sauerborn R, Gbangou A, Dong H, Przyborski JM, Lanzer M. (2005) Willingness to pay for hypothetical malaria vaccines in rural Burkina Faso. Scand J Public Health. 33(2):146-50.

Przyborski J, Lanzer M. (2004) Parasitology. The malarial secretome. Science. Dec 10;306(5703):1897-8.

Przyborski JM, Wickert H, Krohne G, Lanzer M. (2003) Maurer's clefts--a novel secretory organelle?
Mol Biochem Parasitol. Nov;132(1):17-26.

Andrews KT, Pirrit LA, Przyborski JM, Sanchez CP, Sterkers Y, Ricken S, Wickert H, Lepolard C, Avril M, Scherf A, Gysin J, Lanzer M. (2003) Recovery of adhesion to chondroitin-4-sulphate in Plasmodium falciparum varCSA disruption mutants by antigenically similar PfEMP1 variants. Mol Microbiol. 2003 Aug;49(3):655-69.

Przyborski JM, Bartels K, Lanzer M, Andrews KT. (2003) The histone H4 gene of Plasmodium falciparum is developmentally transcribed in asexual parasites. Parasitol Res. 2003 Aug;90(5):387-9.

Reviewing

Molecular and Biochemical Parasitology, Molecular Microbiology, Trends in Parasitology, PLoS ONE, Malaria Journal

Lab life

Apart from doing brilliant science, we also welcome all potential students to engage in our "advanced studies" courses in:

Statistical casino analysis for biologists (winter semester only)

and the very popular

Advanced organic sample preparation for life scientists (summer semester only, weather permitting)