Main Content

Publications

2020

Donati S, Kuntz M, Pahl V, Farke N, Beuter D, Glatter T, Gomes-Filho JV, Randau L, Wang C-Y, Link H (2020) Multi-omics analysis of CRISPRi-knockdowns identifies mechanisms that buffer decreases of enzymes in E. coli metabolism. Cell Syst (in press)

Lu W-T, Trost CN, Müller-Esparza H, Randau L, Davidson AR (2020) An anti-CRISPR protein induces strong non-specific DNA binding activity in a CRISPR-Cas complex. bioRxiv 2020.05.28.119941

Müller-Esparza H, Osorio-Valeriano M, Steube N, Thanbichler M, Randau L (2020) Biolayer interferometry analysis of the target binding activity of CRISPR-Cas effector complexes.
Front Mol Biosci https://doi.org/10.3389/fmolb.2020.00098.

Pinilla-Redondo R, Mayo-Muñoz D, Russel J, Garrett RA, Randau L, Sørensen SJ, Shah SA. (2020)  
Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Res, 48, 2000-2012

Maier LK, Marchfelder A, Randau L (2020)
Meeting report: German Genetics Society - Genome Editing with CRISPR. Bioessays, 42, 2:e1900223. doi: 10.1002/bies.201900223.

2019

Gleditzsch D, Pausch P, Müller-Esparza H, Özcan A, Guo X, Bange G, Randau L. (2019)
PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures. RNA Biol. 16, 504-517. doi: 10.1080/15476286.2018.1504546. 

Turkowyd B, Müller-Esparza H, Climenti V, Steube N, Endesfelder U, Randau L. (2019)
Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance. Methods Enzymol. 2019; 616:133-171. doi: 10.1016/bs.mie.2018.11.001.

Gomes-Filho JV and Randau, L (2019)
RNA stabilization in hyperthermophilic archaea. Ann N Y Acad Sci. 2019 Apr 17. doi: 10.1111/nyas.14060

Yu, N., Jora, M., Solivio, B., Thakur, P., Acevedo-Rocha, C.G., Randau, L., de Crécy-Lagard, V., Addepalli, B., Limbach, P.A. (2019)
Transfer RNA Modification Profiles and Codon Decoding Strategies in Methanocaldococcus jannaschii. J Bacteriol. 2019 Apr 9;201(9). pii: e00690-18. doi: 10.1128/JB.00690-18.

Turkowyd, B., Müller-Esparza, H., Climenti, V., Steube, N., Endesfelder, U. and Randau. L. (2019)
Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance. Methods Enzymol. 616:133-171, doi: 10.1016/bs.mie.2018.11.001

Özcan, A., Pausch, P., Linden, A., Wulf, A., Schühle, K., Heider, J., Urlaub, H., Heimerl, T., Bange, G. and Randau, L. (2019)
Type IV CRISPR RNA processing and effector complex formation in Aromatoleum aromaticum. Nature Microbiol. 4, 89-96, doi: 10.1038/s41564-018-0274-8

2018

Beuter D, Gomes-Filho JV, Randau L, Díaz-Pascual F, Drescher K, Link H. (2018)
Selective Enrichment of Slow-Growing Bacteria in a Metabolism-Wide CRISPRi Library with a TIMER Protein. ACS Synth Biol. 2018 Dec 21;7(12):2775-2782. doi: 10.1021/acssynbio.8b00379

Buddeweg A, Daume M, Randau L, Schmitz RA. (2018)
Noncoding RNAs in Archaea: Genome-Wide Identification and Functional Classification. Methods Enzymol. 2018;612:413-442. doi: 10.1016/bs.mie.2018.08.003. 

Gomes-Filho JV, Daume M, Randau L. (2018)
Unique Archaeal Small RNAs. Annu Rev Genet. 2018 Nov 23;52:465-487. doi: 10.1146/annurev-genet-120417-031300. 

Müller-Esparza, H., Gleditzsch, D. and Randau, L. (2018)
Vielfältige Genscheren: Natürliche Aktivitäten von CRISPR-Cas-Systemen. Biospektrum [German, in press]

Orell, A., Tripp, V., Aliaga-Tobar, V., Albers, S.-V., Maracaja-Coutinho, V., Randau, L. (2018)
A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius. Nucleic Acids Res. 18, 4794-4806.

2017

Daume, M., Uhl, M. Backofen, R. and Randau, L. (2017) 
RIP-Seq suggests translational regulation by L7Ae in Archaea. mbio, 8, pii: e00730-17

Pausch, P., Müller-Esparza, H., Gleditzsch, D., Altegoer, F., Randau, L.*, Bange, G.* (2017) 
Structural variation of type I-F CRISPR RNA guided DNA surveillance. Mol Cell, 67, 622-632. *co-corresponding authors

Randau, L. (2017) 
Two modes of a DNA scissor. Nature Microbiol. 25, 17049 [News & Views]

Orell, A., Schopf, S., Randau, L. and Vera, M. (2017) 
Biofim lifestyle of thermophilic and acidophilic archaea. Biocommunication of Archaea - Springer (ed. Guenther Witzany) [in press]

Richter, H.*, Rompf, J.*, Wiegel, J., Rau, K. and Randau, L. (2017) (*Equal contribution)
Fragmentation of the CRISPR-Cas Type I-B signature protein Cas8b. BBA - General Subjects, pii: S0304-4165(17)30080-6

Müller-Esparza, H. and Randau, L. (2017)
Comment: Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity. Front Microbiol, doi: 10.3389/fmicb.2017.00319

Tripp, V. and Randau, L. (2017)
Evolution of C/D box sRNAs. Nucleic Acids and Molecular Biology - Springer (ed. Béatrice Clouet d'Orval)

until 2016

Tripp, V., Martin, R., Orell, A., Alkhnbashi, O.S., Backofen, R. and Randau, L. (2016)
Plasticity of archaeal C/D box sRNA biogenesis. Mol Microbiol, 103, 151-164.

Gleditzsch, D., Müller-Esparza, H., Pausch, P., Sharma, K., Dwarakanath, S., Urlaub, H., Bange, G. and Randau, L. (2016)
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system. Nucleic Acids Res, 44, 5872-82.

Shao, Y., Richter, H., Sharma, K., Urlaub, H., Randau, L. and Li, H. (2016)
A Non Stem-loop CRISPR RNA Is Processed by Dual Binding Cas6. Structure, 24, 547-554.

Plagens, A., Daume, M., Wiegel, J. and Randau, L. (2015)
Circularization restores signal recognition particle RNA functionality in Thermoproteus. eLIFE, 10.7554/eLife.11623

Dwarakanath, S., Brenzinger, S., Gleditzsch, D., Plagens, A., Klingl, A., Thormann, K. and Randau, L. (2015)
Interference activity of a minimal Type I CRISPR-Cas system from Shewanella putrefaciens. Nucleic Acids Res, 43, 8913-8923.

Dennis, P., Tripp, V., Lui, L, Lowe, T. and Randau, L (2015)
C/D box sRNA-guided 2'-O-methylation patterns of archaeal rRNA molecules. BMC Genomics, 16:632.

Sharma, K., Hrle, A., Kramer, K., Sachsenberg, T., Staals, R.H., Randau, L., Marchfelder, A., van der Oost, J., Kohlbacher, O., Conti, E. and Urlaub H. (2015)
Analysis of protein-RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry. Methods, 89, 138-148.

Plagens, A., Richter, H., Charpentier, E. and Randau L. (2015)
DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes. FEMS Microbiol Rev, 39, 442-463.

Randau, L. (2015)
Evolution of small guide RNA genes in hyperthermophilic archaea. Ann N Y Acad Sci, 1341, 188-193.

Plagens, A. and Randau, L. (2015)
In vitro co-reconstitution of Cas protein complexes. Methods Mol Biol, 1311, 23-33.

Daume, M. Plagens, A. and Randau, L. (2014)
DNA binding properties of the small Cascade subunit Csa5. PLOS ONE. 9:e105716

Daume, M. and Randau, L. (2014)
RNA im Kochtopf (German).Biospektrum. 20, 615-617

Babski, J., Maier,L.-K., Heyer, R., Jaschinski, K., Prasse, D., Jäger, D., Randau, L., Schmitz, R.A., Marchfelder, A. and Soppa, J. (2014)
Small regulatory RNAs in Archaea. RNA Biology, [in press]

Plagens, A., Tripp, V., Daume, M., Sharma, K., Klingl, A., Hrle, A.,Conti, E., Urlaub, H. and Randau, L. (2014)
In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex. Nucleic Acids Res. 42, 5125-5138.

Brendel, J., Stoll, B., Lange, S.J., Sharma, K., Lenz, C., Stachler, A.-E-, Maier, L.-K., Richter, H., Nickel, L., Schmitz, R.A., Randau, L., Allers, T., Urlaub, H., Backofen, R. and Marchfelder, A. (2014)
A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of crRNAs in Haloferax volcanii. J Biol Chem, 289, 7164-7177

Plagens, A. and Randau L. (2014)
Small RNA-guided adaptive immunity: Comment on "Diversity, evolution, and therapeutic applications of small RNAs in prokaryotic and eukaryotic immune systems" by Cooper and Overstreet. Phys Life Rev, 11, 139-140

Hrle, A.*, Su, A.A.H.*, Ebert, J., Benda, C., Randau, L.* and Conti, E.* (2013)
Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3. RNA Biol, 10, 1670-1678 (*joint first/corresponding authorship)

Zöphel, J and Randau, L. (2013)
RNA-Seq analyses reveal CRISPR RNA processing and regulation patterns. Biochem Soc Trans, 41, 1459-1463

Richter, H., Randau, L. and Plagens, A. (2013)
Exploiting CRISPR/Cas: Interference Mechanisms and Applications. Int J Mol Sci, 14, 14518-14531

Su, A.A.H, Tripp, V. and Randau, L. (2013)
RNA-Seq analyses reveal the order of tRNA processing events and the maturation of C/D box and CRISPR RNAs in the hyperthermophile Methanopyrus kandleri. Nucleic Acids Res, 41, 6250-6258

Richter, H., Lange, S.J., Backofen, R. and Randau, L. (2013)
Comparative analysis of Cas6b processing and CRISPR RNA stability. RNA Biol, 10, 700-707

Richter, H., Mohr, S. and Randau, L. (2013)
C/D box sRNA, CRISPR RNA and transfer RNA processing in an archaeon with a minimal, fragmented genome. Biochem Soc Trans, 41, 411-415

Richter, H., Zoephel, J., Schermuly, J., Maticzka, D., Backofen, R. and Randau, L. (2012)
Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis, Nucleic Acids Res, 40, 9887-9896

Randau, L. (2012)
RNA processing in the minimal organism Nanoarchaeum equitans, Genome Biol, 13:R63

Marchfelder, A., Fischer, S., Brendel, J., Stoll, B., Maier, L., Jäger, D., Prasse, D., Plagens, A., Schmitz, R.A., and Randau, L. (2012)
Small RNAs for Defence and Regulation in Archaea, Extremophiles, 16, 685-696

Plagens, A., Tjaden, B., Hagemann, A. Randau, L., Hensel, R. (2012)
Characterization of the CRISPR/Cas I-A system of the hyperthermophilic Crenarchaeon Thermoproteus tenax. J Bacteriol, 194, 2491-500

Zöphel, J., Dwarakanath, S., Richter, H., Plagens, A. and Randau, L. (2012)
Substrate generation for endonucleases of CRISPR/Cas systems. J Vis Exp, 67, doi:pii: 4277

Su, A.A.H., Randau, L. (2011)
A-to-I and C-to-U editing within transfer RNAs. Biochemistry (Moscow), 76, 932-937

Yuan, J., Gogakos, T., Babina, A.M., Söll D. and Randau, L. (2011)
Change of tRNA identity leads to a divergent orthogonal histidyl-tRNA synthetase/tRNAHis pair. Nucleic Acids Res, 39, 2286-2293

Heinemann, I.U., Randau, L., Tomko, R.J. Jr. and Söll D. (2010)
3'-5' tRNAHis guanylyltransferase in bacteria. FEBS Lett, 584, 3567-3572.

Heinemann, I.U., Söll, D. and Randau, L. (2010)
Transfer RNA processing in archaea: Unusual pathways and enzymes. FEBS Lett, 584, 303-309

Heinemann, I.U., O'Donoghue, P., Madinger, C., Benner, J., Randau, L., Noren, C. and Söll, D. (2009)
The appearance of pyrrolysine in tRNAHis guanylyltransferase by neutral evolution. Proc Natl Acad Sci USA, 106, 21103-21108

Mitchell, M., Xue S., Erdman, R., Randau, L., Söll, D. and Li H. (2009)
Crystal structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease. Nucl Acids Res, 37, 5793-5802

Randau, L., Stanley, B.J., Kohlway, A., Mechta, S., Xiong, Y. and Söll, D. (2009)
A cytidine deaminase edits C-to-U in transfer RNAs in archaea. Science, 324, 657-659

Randau, L. and Söll, D. (2008)
Response to "tRNA genes in pieces are an ancestral character". EMBO Rep, 9, 820-821

Randau, L. and Söll, D. (2008)
tRNA genes in pieces. EMBO Rep, 9, 623-628

Randau, L., Schröder, I and Söll, D. (2008)
Life without RNase P. Nature, 453, 120-123

Elkins, J.G., Podar, M., Graham, D.E., Makarova, K.S., Wolf, Y., Randau, L., Hedlund, B.P., Brochier Armanet, C., Kunin, V., Anderson, I., Lapidus, A., Goltsman, E., Berry, K., Koonin, E.V., Hugenholtz, P., Kyrpides, N., Wanner, G., Richardson, P., Keller, M. and Stetter, K.O. (2008)
A korarchaeal genome reveals insights into the evolution of the Archaea. Proc Natl Acad Sci USA, 105, 8102-8107

Polycarpo, C.R., Sheppard, K., Randau, L., Ambrogelly, A., Cardoso, A.M., Fukai, S., Herring, S., Hohn, M.J., Nakamura, Y. and Söll, D. (2006)
Features of Aminoacyl-tRNA Synthesis Unique to Archaea. In: R. Cavicchioli. (ed). Archaea: Molecular Cell Biology. 1 ed. Washington, DC: American Society of Microbiology, pp. 198-208

Randau, L., Calvin, K., Hall, M., Yuan, J., Podar, M., Li, H. and Söll, D. (2005)
The heteromeric Nanoarchaeum equitans splicing endonuclease cleaves non-canonical bulge-helix-bulge motifs of joined tRNA halves. Proc Natl Acad Sci USA, 102, 17934-17939

Randau, L., Pearson, M. and Söll, D. (2005)
The complete set of tRNA species in Nanoarchaeum equitans. FEBS Lett, 579, 2945-2947

Randau, L., Münch, R., Hohn, M.J., Jahn, D. and Söll, D. (2005)
Nanoarchaeum equitans creates functional tRNA from separate genes encoding their 5'- and 3'-halves. Nature, 433, 537-541

Feng, L., Sheppard, K., Namgoong, S., Ambrogelly, A., Polycarpo, C., Randau, L., Tumbula-Hansen, D. and Söll, D. (2004)
Aminoacyl-tRNA Synthesis by Pre-Translational Amino Acid Modification. RNAbiology, 1, 16-20

Randau, L., Schauer, S., Ambrogelly, A., Salazar, J.C., Moser, J., Sekine, S., Yokoyama, S., Söll, D. and Jahn, D. (2004)
tRNA recognition by glutamyl-tRNA reductase. J Biol Chem, 279, 34931-34937