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Electron Microscopy

Dr. Thomas Heimerl

Cell Biology & Electron Microscopy, AG Prof. Uwe-G. Maier
Karl-von-Frisch-Str. 14, 35032 Marburg
Phone: +49-6421 28 21509
Mail:

Description

The core facility for Electron Microscopy is integrated as part of the Laboratory for Cell Biology of Prof. Dr. Uwe-G. Maier. For different kind of samples, various preparation methods and techniques can be offered and applied. Beside classical methods like negative staining of cell suspensions or purified proteins, which is a straight forward technique, some more complex procedures are also performed:

Freeze-etching and freeze-fracturing for the illustration of cell surfaces (BALZER freeze-etching unit),

Chemical fixation and resin embedding of cells,

Cryo-fixation via plunge-freezing for preservation of cellular ultrastructure,

High-pressure freezing of cells and tissues (Wohlwend HPF Compact 02),

Ultrathin sectioning of resin embedded samples (Leica EM UC7rt, Reichert Ultracut-E),

Immunogold localisation of proteins,

Sample preparation for scanning electron microscopy (SEM),

3-D reconstruction via electron tomography,

Post-processing of data sets.

Therefore, it should be possible to find the best fitting method for the specific research interest and finally investigate the samples in a 200 kV Transmission Electron Microscope from JEOL (JEM-2100).
The core facility has been conducted by Dr. Andreas Klingl who changed to the LMU Munich in July 2014.

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Recent Publications

Özcan A, Pausch P, Linden A, Wulf A, Schühle K, Heider J, Urlaub H, Heimerl T, Bange G, Randau L (2019) Type IV CRISPR RNA processing and effector complex formation in Aromatoleum aromaticum. Nature Microbiology 4: 89-96

Dragoš A, Martin M, Garcia CF, Kricks L, Pausch P, Heimerl T, Horváth B, Maróti G, Bange G, López D, Lieleg O, Kovács ÁT (2018) Collapse of genetic division of labor and evolution of autonomy in pellicle biofilms. Nature Microbiology 3: 1451-1460

Bänfer S, Schneider D, Dewes J, Strauss MT, Freibert SA, Heimerl T, Maier UG, Elsässer HP, Jungmann R, Jacob R (2018) A molecular mechanism to recruit galectin-3 into multivesicular bodies for polarized exosomal secretion. Proceedings of the National Academy of Sciences USA 115 :E4396-E4405