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Electron Microscopy

Dr. Thomas Heimerl

Cell Biology & Electron Microscopy, AG Prof. Uwe-G. Maier
Karl-von-Frisch-Str. 14, 35032 Marburg
Phone: +49-6421 28 21509
Mail:

Description

The core facility for Electron Microscopy is integrated as part of the Laboratory for Cell Biology of Prof. Dr. Uwe-G. Maier. For different kind of samples, various preparation methods and techniques can be offered and applied. Beside classical methods like negative staining of cell suspensions or purified proteins, which is a straight forward technique, some more complex procedures are also performed:

Freeze-etching and freeze-fracturing for the illustration of cell surfaces (BALZER freeze-etching unit),

Chemical fixation and resin embedding of cells,

Cryo-fixation via plunge-freezing for preservation of cellular ultrastructure,

High-pressure freezing of cells and tissues (Wohlwend HPF Compact 02),

Ultrathin sectioning of resin embedded samples (Leica EM UC7rt, Reichert Ultracut-E),

Immunogold localisation of proteins,

Sample preparation for scanning electron microscopy (SEM),

3-D reconstruction via electron tomography,

Post-processing of data sets.

Therefore, it should be possible to find the best fitting method for the specific research interest and finally investigate the samples in a 200 kV Transmission Electron Microscope from JEOL (JEM-2100).
The core facility has been conducted by Dr. Andreas Klingl who changed to the LMU Munich in July 2014.

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