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Protein Biochemistry and Spectroscopy

Dr. Oliver Stehling

AG Prof. Roland Lill
Karl-von-Frisch-Straße 14, 35032 Marburg
Phone: +49 6421 28-64044
Mail:

Prof. Dr. Roland Lill

Institute for Cytopathology
Karl-von-Frisch-Straße 14, 35032 Marburg
Phone: +49 6421 28-66449
Mail:

Description

The core facility for protein spectroscopy in the Institute of Cytobiology provides high quality spectrometers, anaerobic facilities, stopped-flow and chromatography equipment. Applications and expertise are focussed on proteins with cofactors and on metalloproteins for which we have extensive experience (see publications below). We support all students and faculty of the Philipps University and their collaboration partners outside our university.

Major instrumentation

  • Jasco FP-6300 Fluorimetry
  • Jasco J-815 CD-Spectrometry
  • Biologic SFM-20 (Stopped flow kinetics)
  • Bruker EMX-6/1 (EPR spectroscopy)
  • Nanotemper Prometheus NT.48, Differential Scanning Fluorimeter (DSF)
  • Nanotemper Monolith NT.115, Microscale-Thermophoresis (MST)
  • Pall ForteBio Octet K2, Biolayer Interferometry (BLI)
  • 4 Anaerobic tents (one with ÄKTA for anaerobic protein purification)
  • Analytical HPLC (Waters/Raytest) with diode array detector
  • 4 Tecan multiplate readers (absorption, fluorescence, luminescence, fluorescence polarisation)
  • Becton Dickinso FACSCanto II flow cytometer
  • Berthold Sirius L – tube luminometer

more details under:
https://www.uni-marburg.de/en/fb20/departments/cyto/core-facilities/core-facility-1

Cooperation Partners