Hauptinhalt

Standort C

Zeiss Spinning disc microscope with extra Rapp UV laser box (CLSM)

·       Axio Observer Z1, inverse, motorized XY, 10 nm focus steps, Z-piezo with 500 µm range, differential interference contrast (DIC), incubator box (temperature, CO2)

·       Yokogawa disc with variable speed (1500–9000 rpm)

·       Solid state laser 405nm, 488nm, 561nm, 639nm

·       emCCD (Photometrics evolve), CCD (AxioCam MRm)

·       Autofocus, focus control in longterm imaging

·       Second independent scan module (Rapp UGA-42 caliburn) for illumination via epiport

·       UV laser box: 355/42 nm (pulsed), 375/70 nm (continuous wave), 473 nm (continuous wave),

·       Microinjection set up with femto jet (Eppendorf) and micromanipulator, Sutter needle puller

• Inhalt ausklappen Inhalt einklappen Objectives

  ·       10x/NA 0.45, 25x/multi/NA 0.8

  ·       40x/water NA 1.1 W

  ·       63x/multi/NA 1.3

  ·       63x/oil/NA 1.4

  ·       100x/oil/NA 1.4

• Inhalt ausklappen Inhalt einklappen Special applications

  ·       Longterm imaging (up to days)

  ·       Fast time-lapse imaging (>20 Hz)

  ·       FRAP

  ·       Ca2+ imaging

  ·       Laser ablation with pulsed UV laser, junctions cuts, wounding, organelle ablation, microsurgery

  ·       UV uncaging with 375 nm UV laser, e.g. NP-EGTA für Ca2+ uncaging

Funding: