Oviedo-Bocanegra LM, Hinrichs R, Rotter DAO, Dersch S, *Graumann PL. (2022) Single molecule/particle tracking analysis program SMTracker 2.0 reveals different dynamics of proteins within the RNA degradosome complex in Bacillus subtilis. Nucleic Acids Research 49:e112-e2112. doi: 10.1093/nar/gkab969
Hernández-Tamayo R, Steube N, Heimerl T, Hochberg GKA, *Graumann PL. (2022) ATPase Activity of Bacillus subtilis RecA Affects the Dynamic Formation of RecA Fila-ments at DNA Double Strand Breaks. mSphere 7:e00412-22. doi: 10.1128/msphere.00412-22
Rotter DAO, Heger C, Oviedo-Bocanegra LM, *Graumann PL. (2021) Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm. BMC Biology 19:183. doi: 10.1186/s12915-021-01083-4
Hernández-Tamayo R, Oviedo-Bocanegra LM, Fritz G, *Graumann PL. (2019) Symmetric activity of DNA polymerases at and recruitment of exonuclease ExoR and of PolA to the Bacillus subtilis replication forks. Nucleic Acids Research 47:8521-8536. doi: doi.org/10.1093/nar/gkz554
Schibany S, Kleine Borgmann LAK, Rösch TC, Knust T, Ulbrich MH, *Graumann PL. (2018) Single molecule tracking reveals that the bacterial SMC complex moves slowly relative to the diffusion of the chromosome. Nucleic Acids Research 46:7805-7819. doi: 10.1093/nar/gky581
Lucena D, Mauri M, Schmidt F, Eckhardt B, *Graumann PL. (2018) Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns. BMC Biology 16:97. doi: 10.1186/s12915-018-0561-0
Kleine Borgmann LA, Ries J, Ewers H, *Ulbrich MH, *Graumann PL. (2013) The bacterial SMC complex displays two distinct modes of interaction with the chromosome. Cell Reports 3:1483-92. doi: 10.1016/j.celrep.2013.04.005
Defeu Soufo HJ, Reimold C, Linne U, Knust T, Gescher J, *Graumann PL (2010) Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Proceedings of the National Academy of Sciences USA 107:3163-8. doi: 10.1073/pnas.0911979107
Soufo CD, Soufo HJ, Noirot-Gros MF, Steindorf A, Noirot P, *Graumann PL. (2008) Cell-cycle-dependent spatial sequestration of the DnaA replication initiator protein in Bacillus subtilis. Developmental Cell. 15:935-41. doi: 10.1016/j.devcel.2008.09.010
Kidane D, *Graumann PL. (2005) Intracellular protein and DNA dynamics in competent Bacillus subtilis cells. Cell. 122:73-84. doi: 10.1016/j.cell.2005.04.036
Selected additional publications
Guo X, Sanchez-Londono M, Gomes-Filho JV, Hernández-Tamayo R, Rust S, Immel-man L, Schäfer P, Wiegel J, Graumann P, Randau L. (2022) Characterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans. Nature Microbiology 2022 7:1870-1878. doi: 10.1038/s41564-022-01229-2
Krol E, Stuckenschneider L, Kästle J, Graumann PL, Becker A (2021) Stable inheritance of Sinorhizobium meliloti cell growth polarity requires an FtsN-like protein and an amidase. Nature Communications 12:545, doi: 10.1038/s41467-020-20739-3.
R. Hernandez-Tamayo, H. Schmitz, PL Graumann, Single molecule dynamics at a bacterial replication fork after nutritional downshift or chemically induced block in replication, mSphere, (2021), 6, e00948-20
Simon Dersch, Johanna Mehl, Lisa Stuckenschneider, Benjamin Mayer, Julian Roth, Alexander Rohrbach, Peter L Graumann (2020) Super-resolution microscopy and single-molecule tracking reveal distinct adaptive dynamics of MreB and of cell wall-synthesis enzymes. Front. Microbiol. 11:1946 doi: 10.3389/fmicb.2020.01946
Nina El Najjar, David Geisel, Felix Schmidt, Simon Dersch, Benjamin Mayer, Raimo Hartmann, Bruno Eckhardt, Peter Lenz, Peter L. Graumann (2020) Chromosome segregation in B. subtilis follows an overall pattern of linear movement and is highly robust against cell cycle perturbations. mSphere 5:e00255-20 doi: 10.1128/mSphere.00255-20
Kunz S, Tribenski A, Steinchen W, Bedrunka P, Graumann PL (2020) Cyclic-di-GMP signaling in Bacillus subtilis is governed by direct interactions of diguanylate cyclases and cognate receptors. mBio 11
Bughard-Schrod M, Altenburger S, Graumann PL (2020) The Bacillus subtilis dCMP deaminase ComEB acts as a dynamic polar localization factor for ComGA within the competence machinery. Mol. Microbiol. 113:906-922
Hernández-Tamayo R, Oviedo Bocanegra L, Fritz G, Graumann PL (2019) Symmetric activity of DNA polymerases at and recruitment of exonuclease ExoR and of PolA to the Bacillus subtilis replication forks. Nucleic Acids Res. 47:8521-8536
Rösch TC, Altenburger S, Oviedo-Bocanegra L, Pediaditakis M, Najjar NE, Fritz G, Graumann PL. (2018) Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres. Sci Rep. 2018 Nov 6;8(1):16450. doi: 10.1038/s41598-018-34572-8.
Rösch TC, Oviedo-Bocanegra LM, Fritz G, Graumann PL. (2018) SMTracker: a tool for quantitative analysis, exploration and visualization of single-molecule tracking data reveals highly dynamic binding of B. subtilis global repressor AbrB throughout the genome. Sci Rep. 2018 Oct 24;8(1):15747. doi: 10.1038/s41598-018-33842-9.
Schibany S, Kleine Borgmann LAK, Rösch TC, Knust T, Ulbrich MH, Graumann PL. (2018) Single molecule tracking reveals that the bacterial SMC complex moves slowly relative to the diffusion of the chromosome. Nucleic Acids Res. 2018 Sep 6;46(15):7805-7819. doi: 10.1093/nar/gky581.
Lucena D, Mauri M, Schmidt F, Eckhardt B, Graumann PL. (2018) Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns. BMC Biol. 2018 Sep 3;16(1):97. doi: 10.1186/s12915-018-0561-0.
Schenk K, Hervás AB, Rösch TC, Eisemann M, Schmitt BA, Dahlke S, Kleine-Borgmann L, Murray SM, Graumann PL (2017) Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication. PLoS Genet. 13: e1006561.
Dempwolff F, Schmidt FK, Hervás AB, Stroh A, Rösch TC, Riese CN, Dersch S, Heimerl T, Lucena D, Hülsbusch N, Stuermer CA, Takeshita N, Fischer R, Eckhardt B, Graumann PL (2016) Super Resolution Fluorescence Microscopy and Tracking of Bacterial Flotillin (Reggie) Paralogs Provide Evidence for Defined-Sized Protein Microdomains within the Bacterial Membrane but Absence of Clusters Containing Detergent-Resistant Proteins. PLoS Genet. 12(6):e1006116
Defeu Soufo HJ, Reimold C, Breddermann H, Mannherz HG, Graumann PL (2015) Translation Elongation Factor EF-Tu Modulates Filament Formation of Actin-Like MreB Protein In Vitro. J. Mol. Biol. 427:1715-1727
Rösch, T Graumann, P L (2015) Induction of Plasmid Conjugation in Bacillus subtilis Is Bistable and Driven by a Direct Interaction of a Rap/Phr Quorum-sensing System with a Master Repressor. J. Biol. Chem. 290: 20221-20232
El Andari, J, Altegoer, F, Bange, G, Graumann PL (2015) Bacillus subtilis bactofilins are essential for flagellar hook- and filament assembly and dynamically localize into structures of less than 100 nm diameter underneath the cell membrane. PLOS One 10:e0141546
Rösch TC, Golman W, Hucklesby L, Gonzalez-Pastor JE, Graumann PL (2014) The presence of conjugative plasmid pLS20 affects global transcription of its Bacillus subtilis host and results in beneficial stress resistance to cells. Appl. Environ. Microbiol. 80:1349-1358
Völker T, Dempwolff F, Graumann PL, Meggers E. (2014) Progress towards Bioorthogonal Catalysis with Organometallic Compounds. Angew. Chem. Int. Ed. Engl. 53, 10536-10540
Kleine-Borgmann, LA, and Graumann, PL (2014) Structural maintenance of chromosomes (SMC) complex in bacteria. J. Mol. Microbiol. Biotechnol. 24: 384-395
Kleine-Borgmann L.A., Ries J., Ewers H., Ulbrich M.H., Graumann P.L. (2013) The bacterial SMC complex displays two distinct modes of interaction with the chromosome. Cell Rep. 3:1483-1492
Reimold, C., H.-J. Defeu Soufo, F. Dempwolff and P. L. Graumann (2013) Motion of variable-length MreB filaments at the bacterial cell membrane influences cell morphology. Mol. Biol. Cell 24: 2340-2349
v. Olshausen P., H. J. Defeu Soufo, K. Wicker, R. Heintzmann, P. L. Graumann, and A. Rohrbach. (2013) Superresolution Imaging of Dynamic MreB Filaments in B. subtilis — A Multiple-Motor-Driven Transport? Biophysical Journal 105:1171-1181
Dempwolff F, Möller HM, Graumann PL. (2012) Synthetic Motility and Cell Shape Defects Associated with Deletions of Flotillin/Reggie Paralogs in Bacillus subtilis and interplay of these proteins with NfeD proteins J. Bacteriol. 194:4652-4661
Cárdenas PP, Carrasco B, Defeu Soufo C, César CE, Herr K, Kaufenstein M, Graumann PL, Alonso JC. (2012) RecX facilitates homologous recombination by modulating RecA activities. PLOS Genet. 8:e1003126
Pediaditakis, M., M. Kaufenstein and P. L. Graumann (2012) Bacillus subtilis hlpB encodes a conserved stand-alone HNH nuclease-like protein that is essential for viability unless the hlpB deletion is accompanied by the deletion of genes encoding the AddAB DNA repair complex. J. Bacteriol. 194:6184-6194
Kaufenstein M., van der Laan M., and P. L. Graumann (2011) The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex. J. Bacteriol. 193:1633-1642
Bauer T, Rösch T, Itaya M, Graumann PL. (2011) Localization pattern of conjugation machinery in a Gram-positive bacterium. J. Bacteriol. 193:6244-6256
Jockusch BM, Graumann PL. (2011) The long journey: actin on the road to pro- and eukaryotic cells. Rev. Physiol. Biochem. Pharmacol. 161:67-85.
Graumann, P. L. (2011) Chromosome segregation. In “Cellular and molecular biology of Bacillus subtilis. Ed. Peter L. Graumann, Horizon Scientific Press, Norfolk, UK , 2nd edition, p
Kaimer C, Graumann PL. (2011) Players between the worlds: multifunctional DNA translocases. Curr. Opin. Microbiol. 14:719-725
Krishnamurthy, M., S. Tadesse and P. L Graumann(2010) A novel SMC-like protein, SbcC2 (YhaN), is involved in DNA double strand break repair and competence in Bacillus subtilis. Nucleic Acids Res. 38, 455-466
Defeu Soufo H. J., C. Reimold, U. Linne, T. Knust, J. Gescher, and P. L. Graumann (2010). Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Proc. Natl. Acad. Sci. U S A 107: 3163-3168.
Defeu Soufo H. J., and Graumann P. L. (2010) Bacillus subtilis MreB paralogues have different filament architectures and lead to shape remodelling of a heterologous cell system. Mol. Microbiol. 78:1145-1158
Kidane, D., B. Carrasco, C. Manfredi, K. Rothmaier, C. Cañas, S. Ayora, S. Tadesse, J. C. Alonso and P. L. Graumann < Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells. PLOS Genetics 5:e1000630
Kaimer, C., J. E. González-Pastor and P. L. Graumann (2009) SpoIIIE and a novel type of DNA translocase, SftA, couple chromosome segregation with cell division in Bacillus subtilis. Mol. Microbiol. 74: 810–825
Waidner, B., M. Specht, F. Dempwolff, K. Häberer, S. Schätzle, V. Speth, M. Kist, P. L. Graumann(2009) A novel system of cytoskeletal elements in the human pathogen Helicobacter pylori. PLOS Pathogens 5:e1000669
Defeu Soufo, C., H. J. Defeu Soufo, M-F. Noirot-Gros, A. Steindorf, P. Noirot and P. L. Graumann (2008) Cell cycle-dependent spatial sequestration of the DnaA replication initiator protein in Bacillus subtilis. Dev. Cell 15, 935–941
Graumann, P. L. (2007) Cytoskeletal elements in bacteria. Annu. Rev. Microbiol. 61:589-618
Tadesse, S. and P. L. Graumann (2006) Differential and dynamic localization of topoisomerases in Bacillus subtilis. J. Bacteriol. 188: 3002-3011
Defeu Soufo, H-J and P. L. Graumann (2006) Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the function of MreB. Mol. Microbiol. 62: 1340-1356
Kidane, D. and P. L. Graumann (2005) Dynamic formation of RecA filaments at DNA double strand break repair centres in live cells. J. Cell. Biol. 170: 357-366
Kidane, D. and P. L. Graumann (2005) Intracellular protein and DNA dynamics in competent Bacillus subtilis cells. Cell 122: 73-84
Defeu-Soufo, H-J. and P. L. Graumann (2004) Dynamic motor like movement of actin proteins within a bacterium. EMBO Reports 5: 789-794
Kidane, D., H. Sanchez, J. Alonso and P. L. Graumann (2004) Visualization of DNA double strand break repair in live bacteria reveals dynamic recruitment of RecF, RecO and RecN proteins to distinct sites on the nucleoids. Mol. Microbiol. 52: 1627-1639
Volkov, A., J, Mascarenhas, C. Andrei-Selmer, H. Ulrich and P. L. Graumann (2003) A prokaryotic condensin/cohesin-like complex can actively compact chromosomes from a single position on the nucleoid. Mol. Cell Biol., 23: 5638-5650
Defeu-Soufo, H-J. and P. L. Graumann (2003) Actin like proteins MreB and Mbl in Bacillus subtilis are required for bipolar positioning of replication origins. Current Biol. 28: 1916-1920
Mascarenhas, J., J. Soppa, A. Strunnikov and P. L. Graumann (2002) Cell cycle dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein. EMBO J. 21: 3108-3118
Mascarenhas, J. , M. H. W. Weber and P. L. Graumann (2001) Specific localization of ribosomes to polar spaces in Bacillus subtilis depends on active transcription. EMBO Reports 2: 685-689