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Equipment

Confocal laser scanning microscopes:

Leica STELLARIS 8 FALCON (Biologiegebäude, Karl-von-Frisch-Str. 8)

·         DMi8 CS inverses Mikroskop mit Super Z Galvo Tisch and Okolab Inkubationsbox

·         Diodenlaser 405 nm, 488 nm; Weißlichtlaser 440 – 790 nm

·         Detektoren: 2 x HyD S, 2 x HyD R, 1 x HyD R (Detektionsbereich 440 – 850 nm)

·         FOV Scanner

  • STELLARIS 8 objectives:

    ·         PL APO 20x/0,75 IMM CORR

    ·         HC FLUOTAR L 25x/0,95 X VISIR 0,17

    ·         PL APO 40x/1,10 W mot CORR

    ·         PL APO 63x/1,30 GLYC CORR DIC

  • STELLARIS 8 applications:

    o         Long-term live cell imaging with temperature and CO2 control

    o           FRAP

    o          FRET

    o           FLIM solutions: TauSense (qualitative) und FALCON (quantitative)

    o          photon counting (HyD X und HyD R)

    o          Navigator

    o           LIGHTNING (deconvolution)

    o          DSE (Dynamic Signal Enhancement)

    o          Lambda Scans

    o           Live Data Mode

Leica TCS SP5 (Biology building, Karl-von-Frisch-Str. 8)

·         DM6000 B upright microscope with Z galvo stage

·         Lasers: UV diode 405 nm; Ar 458, 476, 488, 496, 514 nm; DPSS 561 nm, HeNe 594 nm, HeNe 633 nm

·         Detectors: 5 x PMT (detection range 400 – 800 nm)

·         FOV and resonant scanner

  • SP5 objectives:

    ·         HC PL FLUOTAR 10x/0,30

    ·         HC PL APO 10x/0,40 IMM CS

    ·         HCX PL APO 20x/0,70 lmm Corr Lbd. bl. HCX 

    ·         PL APO 40x/1,25-0,75 Oil Lbd. bl. HCX PL 

    ·         APO 63x/1,40 oil PH3

    ·         HCX PL APO 63x/1,3 GLYC CORR CS 21 

  • SP5 applications:

    · o     Multidimensional recording and analysis of image series

    o       FRAP

    o       FRET

    o       Mark & Find

    o       Lambda Scans

    o        Live Data Mode

Zeiss LSM980 with extra Rapp UV-Box (Multipurpose building MZVG 06C06, Hans-Meerwein-Str.6)

·         Axio Observer, inverse, motorized XY, 10 nm focus steps, Z-piezo with 500 µm range, motorized filter ring, condensor with shutter, black incubator box 

·         Solid state laser 405nm, 488nm, 514nm, 561nm, 594nm, 639nm

·         Airyscan 2 array detector, Spectral GaAsP detector, 2x photomultiplier detector

·         Photoncounting mode 

·         Autofocus, focus control in longterm imaging

·         Second independent scan module (Rapp UGA-42 caliburn)for illumination via epiport 

·         UV laser box: 355/42 nm (pulsed), 375/70 nm (continuous wave)

·         large system table NLO table, 1.5x1.8m

·         second computer, linked to system for real-time data transfer and processing

  • LSM980 objectives:

    ·         C-Apochromat 40x/1.2 W 

    ·         Plan-Apochromat 63x/1.4 oil 

    ·         LCI Plan-Apochromat 63x/1.2 multi immersion

  • LSM980 special applications

    o   Recording based highresolution imaging down to 120 nm lateral

    o   Multiplex imaging (up to eight lines simultaneously)

    o   FRAP

    o   fluctuation analysis and FCS

    o   Laser ablation with pulsed UV laser, junctions cuts, wounding, organelle ablation, microsurgery

    o   UV uncaging with 375 nm UV laser, e.g. NP-EGTA für Ca2+ uncaging

    o   realtime processing and data transfer to second computer

    o   longterm (multiple hours) time lapse imaging with readjusted axial focus

    o   tiling of large objects (mm scale)

Epifluorescence microscopes:

Zeiss AxioObserver Z1 (Biology building, Karl-von-Frisch-Str. 8)

·         Inverted microscope with motorized xyz stage

·         Filters: DAPI, GFP, YFP, CY3, CY5

·         Camera: Zeiss Axiocam 305 mono R2

Zeiss AxioObserver (Multipurpose building MZVG 06C06, Hans-Meerwein-Str.6)

·         AxioObserver Z1, inverted, differential interference contrast, phase contrast, motorized stage with joystick

·         Detectors: AxiocamMR, emCCD (photometrics evolve 512), ApoTome

Zeiss AxioPlan (Multipurpose building MZVG 06C06, Hans-Meerwein-Str.6)

·         Axioplan 2, upright, differential interference contrast, phase contrast, darkfield optics

·         Detectors AxiocamMR, ApoTomeI

Funding: